Figure 5
Antagonism of the CBS inhibitor on the expression and function of P2X3 receptors. (A) Administration of the CBS inhibitor O-(carboxymethyl) hydroxylamine hemihydrochloride (AOAA, 10 μg/kg) significantly reduced the expression of P2X3Rs in L5–L6 DRGs from LDH rats (*P < 0.05, n = 7 for each group). (B) In LDH rats, application of AOAA greatly reduced the ATP-evoked intracellular calcium mobilization when compared to NS-treated rats (NS, n = 48 cells, LDH, n = 56 cells, *P < 0.05, two sample t-test). (C) The percentage of neurons responding to ATP application was not significantly altered in LDH rats when compared to sham rats (n = 96 cells for each group, P > 0.05, χ 2 test). (D) LDH treatment significantly enhanced expression of CBS in L5–L6 DRGs when compared to sham rats (n = 7 for each group, *P < 0.05). (E) There was no significant difference in CBS expression in T10-12 DRGs between the sham and LDH groups (n = 6 for each group). (F) There was no significant difference in CSE expression in L5-6 DRGs between the sham and LDH groups (n = 6 for each group).